Reactive Deep Eutectic Solvents (RDESs): A New Tool for Phospholipase D-Catalyzed Preparation of Phospholipids

The use of Reactive Deep Eutectic Solvents (RDESs) in the preparation polar head modified phospholipids (PLs) with phospholipase D (PLD)-catalyzed biotransformations has been investigated. Natural phosphatidylcholine (PC) submitted to PLD-catalyzed transphosphatidylations using a new reaction medium composed by mixture RDES/buffer. Instead exploiting deep eutectic solvents conventionally, just as media, these have designed here order contribute actively synthetic processes participating reagents. RDESs were prepared choline chloride or trimethyl glycine hydrogen-bond acceptors and glycerol ethylene glycol, donors well nucleophiles for substitution. Specifically RDES/buffer media allowed obtainment PLs optimized yields perspective sustainable process implementation.